KEY FEATURES

Simple Mug Test

This is a simple reliable technique which uses a dry medium to enable the rapid confirmed enumeration of both total coliforms and E.coli.

The media turns blue-green in the presence of coliform bacteria (as illustrated in the above photo). E. coli growth is confirmed by blue fluorescence under UV light or using a Kovacs reagent.

The sample should be incubated for 24 hours at 35 deg C or 48 hours at 25 deg C. After the 24 hour or 48 hour incubation time observe the colour of the sample.

For Coliform Confirmation:

a) CLEAR/YELLOW = NEGATIVE for Coliforms
b) BLUE/GREEN = POSITIVE for Coliforms

For E.Coli Confirmation:

Shine a UV light (approx 365nm) from the bottom of the sample while avoiding looking directly at the light.

a) NO FLUORESCENCE = NEGATIVE for E.Coli
b) BLUE FLUORESCENCE = POSITIVE for E.Coli

If further confirmation of E.Coli is desired add 1ml of Kovac’s Indole reagent (sold separately) to the sample , recap the bottle and wait 30 seconds. A RED ring will form if E.Coli is present.

If results are positive add bleach to the sample before pouring down the toilet.

REAGENT CODES